六盘山华北落叶松林和红桦林枯落物持水特征及其截持降雨过程

在六盘山南坡野外调查了华北落叶松(Larix principis-rupprechtii)和红桦(Betula albo-sinensis)两个林分的枯落物数量特征,其厚度分别是3.6cm和4.0cm,储量为16.83 t/hm2和9.04 t/hm2.浸泡测定了单位重量(1.0kg/m2)枯落物的持水能力,华北落叶松林和红桦林的最大持水深分别是1.8 mm和2.7 mm.在六盘山地区自然雨强范围内(<30 mm/h),利用模拟降雨研究了枯落物的截持降雨过程,表明枯落物持水能力随雨强增大而提高;在雨强为30 mm/h时,华北落叶松林和红桦林枯落物(1.0kg/m2)的最大截持雨量为1.5mm和2.6mm,分别是浸泡测定持水能力的83.3%和96.2%,两种实验吸持水分的过程基本一致.分析表明,枯落物截持过程的吸水速率受其本身含水量大小的影响,同时构建了具有较好截持机制的枯落物截持降雨过程模型,并基于枯落物浸泡实验和模拟降雨截持实验的数据拟合了模型参数.     

河道生态系统特征及其自净化能力研究现状与发展

河道生态系统包括河道水体和河岸带两大部分,具有净化水体、降低污染物浓度的功能.研究河道生态系统的自净化能力对河道生态系统的健康评价及水环境管理具有重要的意义.目前该领域的研究主要侧重于两个方面:一方面是运用水质模型的方法模拟评价河道水体的自净化能力,另一方面则是研究河岸植被缓冲带对净化水体的作用.系统总结了目前研究的特点与不足,指出了今后应该重视的研究领域,主要包括:(1)应加强研究陆地生态系统空间异质性对河道自净化能力的影响研究;(2)需要系统解剖河道剖面形态结构与空间布局对河道水质自净化能力的影响;(3)重视面向外部复杂环境的参数获取方法的研究;(4)综合河岸生态系统与河道水体生态系统,系统研究非点源污染在陆-水迁移和水体运移中河道生态系统自净化能力的时空变化特征,从而为开展河道生态系统健康评价,河道生态系统管理提供科学的依据.     

渝北水源区水源涵养林构建模式对土壤渗透性的影响

土壤渗透性是评价土壤水源涵养作用重要指标之一,其受制于许多外在和内在因素的影响,与植被类型、土壤结构、土壤种类和降雨强度等关系密切。为探讨不同构建模式水源涵养林对土壤渗透性能的影响,以裸地为对照,以土壤初渗速率,稳渗速率,平均渗透速率和渗透总量表征土壤渗透性,对重庆北部水源区8种不同构建模式的水源涵养林地的土壤渗透性及其与理化性质的关系进行研究。结果表明:不同模式水源林地和同一模式林地不同层次土壤渗透性能存在较大差异,各林地土壤渗透性随土壤深度的增加而降低,母质层土壤渗透性受林分类型和林分结构等外界条件的干扰较小,差异不显著;8种林地土壤渗透性均好于裸地,各林地土壤渗透性能大小依次为:广东山胡椒×杉木混交林马尾松×柳杉混交林(火烧迹地)四川大头茶×四川山矾混交林毛竹×四川山矾×马尾松混交林马尾松×广东山胡椒混交林马尾松×四川大头茶混交林广东山胡椒×四川杨桐混交林毛竹纯林裸地,根据土壤渗透能力可将8种模式林分类型分为4类:第1类,广东山胡椒×杉木混交林和马尾松×柳杉混交林,土壤渗透性最好;第2类,四川大头茶×四川山矾混交林,土壤渗透能力较好;第3类,毛竹×四川山矾×马尾松混交林、马尾松×广东山胡椒混交林、广东山胡椒×四川杨桐混交林、马尾松×四川大头茶混交林,土壤渗透性好;第4类,毛竹林和裸露地,土壤渗透性最差。以广东山胡椒和杉木混交的林分模式的土壤渗透性最好,毛竹纯林地最差,毛竹纯林对提高土壤渗透性作用较差,营林时适当增加毛竹林中其他树种的混交比例可增强林地土壤渗透性能。通用经验方程对各林分土壤入渗过程的拟合效果最好,比较适用于描述本研究区域水源涵养林地土壤入渗特征的模型,Kostiakov方程拟合效果次之,Horton方程最差;结合相关分析,筛选出9个极显著或显著影响土壤渗透性能的土壤理化性质因子,通过主成分分析法,得到土壤渗透性能综合参数α及与其极显著相关因子的综合参数β,并构建土壤渗透性各指标及其综合参数α与β的线性回归模型,为重庆北部的水源涵养林建设和防治水土流失提供理论依据。     

蝴蝶兰胚性愈伤组织诱导的氧化还原动态

离体培养蝴蝶兰（Phalaenopsis spp.）类原球茎切块诱导胚性愈伤组织,在培养第11～18 d时出现胚性细胞,第25～32 d观察到幼嫩的球形胚。在培养第0～11 d,外植体.OH相对含量波动不大,O2.生成速率持续提高并达到高峰,H2O2水平明显提高,抗氧化能力在低位波动,推测外植体可能经受较强的氧化胁迫,以利细胞脱分化和获得胚性潜能。此外,此期间SOD活性持续提高,POD和CAT活性达到一个高峰。在愈伤组织诱导全过程中,总抗氧化能力、DPPH自由基清除率、H2O2水平基本上都持续提高,与SOD活性均极显著正相关,SOD是影响培养物清除自由基能力和提高抗氧化水平的主要因素。综合分析实验结果表明,在蝴蝶兰胚性愈伤组织诱导过程中,SOD、O2.和H2O2起到关键的作用。     

Performance characteristics according to Commission Decision 2002/657/EC in the fluorimetric determination of tetracycline in the absence and in the presence of magnesium.

The fluorimetric determination of tetracycline is usually carried out in the presence of some metals that, through the formation of a complex with this antibiotic, enhance its fluorescence emission, giving more sensitive determination methods. It is well established that magnesium is one of these metals. However, it is possible that higher signals do not mean a real improvement in the quality of the analytical method. In this work, the univariate and multivariate fluorescence determination of tetracycline is performed in the presence and absence of Mg(2+), comparing the quality of the analyses through some performance characteristics that, according to Commission Decision 2002/657/EC define the functional qualities of analytical methods. The methods with the best performance characteristics were multivariate determinations carried out in the absence of Mg(2+), both when emission or excitation spectra were taken, the decision limits (CCalpha) being 13.1 and 20.1 microg/L and the detection capabilities (CCbeta) 25.3 and 38.5 microg/L, respectively. This study points out through a case study that higher analytical signals do not necessarily mean better performance characteristics of a method of analysis.     

Supramolecular ligands: Monomer structure and protein ligation capability

The aim of this work was to define the chemical structure of compounds self-assembling in water solutions, which appear to interact with proteins as single ligands with their supramolecular nature preserved. For this purpose the ligation to proteins of his azo dyes, represented by Congo red and its derivatives with designed structural alterations, were tested. The three parameters which characterize the reactivity of supramolecular material were determined in the same conditions for all studied dyes. These were: A) stability of the assembly products; B) binding to heat-denatured protein (human IgG); and C) binding to native protein (rabbit antibodies in the immune complex) measured by the enhancement of hemagglutination. The structural differences between the Congo red derivatives concerned the symmetry of the molecule and the structure of its non-polar component, which occupies the central part of the dye molecule and is thought to be crucial for self-assembly. Other dyes were also studied for the same purpose: Evans blue and Trypan blue, bis-ANS and ANS, as well as a group of compounds with a structural design unlike that of bis azo dyes. Compounds with rigid elongated symmetric molecules with a large non-polar middle fragment are expected to form a ribbon-like supramolecular organization in assembling. They appeared to have ligation properties related to their self-assembling tendency. The compounds with different structures, not corresponding to his azo dyes, did not reveal ligation capability, at least in respect to native protein. The conditions of binding to denatured proteins seem less restrictive than the conditions of binding to native molecules. The molten hydrophobic protein interior becomes a new binding area allowing for complexation of even non-assembled molecules.     

三化螟、二化螟及大螟成虫的飞翔能力

本文利用飞行磨(Flight mill)装置对三化螟Tryporysa in certulas(Walkr) 二化螟 Chilo suppressalis(Walker);及大螟Sesamia infirence(Walker)成虫的飞行能力进行了测试,结 果表明：三化螟与二化螟成虫的飞翔能力基本一致,其有效飞行日龄均为4天,成虫羽化后无明显的生殖前期(<1天),卵巢发育为II级时(1日龄)飞行能力最强。最远累计飞行距离可达32公里以上。雌雄虫具有不同的持续飞翔能力。大螟是这三种螟虫中飞翔能力最强的一种,具有极强的持续飞行能力,雌、雄虫可分别达32与50公里以上,大螟的有效飞行日龄一般为6天,且具有2-3天的生殖前期,卵巢发育为II级时飞翔能力最强,随日龄的增加,飞翔能力逐步下降,大螟成虫具有远距离扩散飞翔能力及一定的迁飞生理行为基础。     

Effects of cooling, cryopreservation and heating on sperm proteins, nuclear DNA, and fertilization capability in mouse

In the present study, we used confocal microscopy and electrophoresis to study the effects of heating to 5 or 100 degrees C or cooling to 4 degrees C or -- 196 degrees C on the stability of sperm proteins and DNA. We used intracytoplasmic sperm injection (ICSI) to determine the fertilizing capability of treated spermatoza. It was shown that sperm cryopreservation at - 196 degrees C or cooling at 4 degrees C altered neither protein and DNA profiles nor the sperm fertilization capability, while the protein and DNA profiles of sperm heated at 100 degrees C were irreversibly degraded and inactivated. The proteins of sperm were severely damaged while the nuclear DNA still maintained its integrity when heated to 58 degrees C. Observation by laser confocal microscopy showed that after being heated to 58 degrees C and 100 degrees C, the nuclear of mouse sperm lost their ability to activate oocytes and they could not transform to male pronuclei though the membrane of some sperm could degrade and induce the formation of sperm asters in ICSI oocytes. The results indicate that the use of 58 degrees C heating only causes the degradation of sperm proteins, while the 100 degrees C heating elicits the irreversible degradation of both sperm proteins and nuclear DNA, and the damage of sperm proteins is primarily responsible for the observed decrease in sperm fertilizing capability.     

Who’s afraid of perfectionist moral enhancement? A reply to Sparrow

Robert Sparrow recently argued that state-driven moral bioenhancement is morally problematic because it inevitably invites moral perfectionism. While sharing Sparrow’s worry about state-driven moral bioenhancement, I argue that his anti-perfectionism argument is too strong to offer useful normative guidance. That is, if we reject state-driven moral bioenhancement because it cannot remain neutral between different conceptions of the good, we might have to conclude that all forms of moral enhancement programs ought not be made compulsory, including the least controversial and most popular state-driven program: compulsory (moral) education. In this paper, I argue that, instead, the spirit of Sparrow’s worry should be recast in the language of the capability approach—an approach that strives to enhance people’s capabilities to develop their own conceptions of the good by restricting itself from endorsing thick conceptions of the good. The distinction made regarding thick and thin conceptions of the good helps to capture sentiments against state-driven bioenhancement programs without falling prey to the issues I raise against Sparrow’s anti-perfectionist arguments.     

紫花苜蓿叶输导组织解剖结构和光合特性对外源氮的响应

以紫花苜蓿品种‘甘农3号’和‘陇东苜蓿’为研究材料,采用室外(防雨网室)盆栽营养液砂培法,研究了2种氮素形态(NO3--N,NH4+-N)的5个氮素水平(0、105、210、315、420mg·L-1)处理对叶片输导组织解剖结构及光合特性的影响。结果显示:(1)与无氮处理相比,供氮处理下‘甘农3号’和‘陇东苜蓿’输导组织的解剖结构和光合特性发生明显变化,叶片输导组织维管束、木质部和韧皮部面积显著增大,导管数显著增多,净光合速率、气孔导度、叶面积和叶绿素含量均显著增加,且在210mg·L-1供氮水平下达到最大值。(2)2种氮素形态相比,‘甘农3号’和‘陇东苜蓿’叶片输导组织维管束面积、木质部面积、韧皮部面积、导管数、净光合速率、气孔导度、叶面积、叶绿素含量均表现为NH4+-N处理好于NO3--N处理。(3)2品种相比,叶片输导组织维管束面积、木质部面积、韧皮部面积、导管数、净光合速率、气孔导度、叶面积、叶绿素含量表现为‘甘农3号’大于‘陇东苜蓿’。研究表明,氮素能通过改善紫花苜蓿叶片输导组织的解剖结构和光合特性,促进紫花苜蓿光合作用;各处理中以NH4+-N、210mg·L-1表现最佳,维管束面积最大,木质部、韧皮部面积大且发育好,导管数最多,而且‘甘农3号’表现更优。